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Comment: DESeq2 analysis with transcript counts by Sink • 0

Your workflow seems good for a beginner! Using nf-core for RNASeq processing and DESeq2 for differential expression analysis is a standard …

Comment: Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) retu by ATpoint ★ 4.6k

Annotation packages have their value. They are a convenient and stable way to make annotations accessible inside R, they are version-contro…

Comment: Installation error for Rsamtools. by ATpoint ★ 4.6k

In other words to what James wrote, the package is already installed, there is nothing you need to do.

Comment: Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) retu by ATpoint ★ 4.6k

Given that the annotation versions were the same the results *should* be identical, but for such a simple task as making a tx2gene why not …

Answer: Differential variability modelling with vst() expression by Gordon Smyth 52k

See Phipson, B., Oshlack, A. DiffVar: a new method for detecting differential variability with application to methylation in cancer and …

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A: Trajectory analysis for single cell sequencing

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